Benomyl resistant mutants of Schizosaccharomyces pombe cold-sensitive for mitosis

Meiotic recombination-deficient mutants of Schizosaccharomyces pombe.

A mutant screen employing the ade6-M26 recombination hotspot was developed and used to isolate Schizosaccharomyces pombe mutants deficient in meiotic recombination. Nine rec mutations were recessive, defining six complementation groups, and reduced ade6 meiotic recombination 3-fold to greater than or equal to 300-fold when homozygous. Three recessive rec mutations analyzed further also reduced ...

Dependency relations between events in mitosis in Schizosaccharomyces pombe.

Hexavalent chromium uptake by sensitive and tolerant mutants of Schizosaccharomyces pombe.

Lysine and leucine auxotrophic, heterothallic (h+, h-) strains of Schizosaccharomyces pombe were used to obtain chromium (VI)-sensitive and -tolerant mutants by ultraviolet radiation-induced and nitrosoguanidine-induced mutagenesis. The minimal inhibitory concentrations of K2Cr2O7 on YEA media were 225 microM for the wild-type strain CW-6, 125 microM for the sensitive mutant CS-6.51 and 275 mic...

Schizosaccharomyces pombe mutants affected in their division response to starvation.

Schizosaccharomyces pombe mutants have been selected on the basis of an altered response to nutritional stimulation of cell division (changed division response, cdr). Two new loci (cdr1 and cdr2) were identified and characterized. When suspended in nitrogen-free medium wild-type cells underwent stimulated rates of division and became reduced to approximately 30% in protein content with a concom...

Schizosaccharomyces pombe mutants that are defective in glycoprotein galactosylation.

Several mutants of Schizosaccharomyces pombe were obtained that are defective in protein glycosylation. One of the mutants, strain Sp550, makes galactomannoproteins with about half of the wild-type amount of galactose, whereas another strain, Sp137, makes glycoproteins that are almost devoid of galactose. Nondenaturing gel electrophoresis of cell extracts of both mutants revealed that they make...